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Antiproliferative effects of JGL2 on human lung cancer cells.
Juniperus glabra L., a plant in the juniper family, is mainly used for its astringent properties. Previous studies have shown that extracts of this plant can inhibit the growth of mouse Ehrlich carcinoma cells in vivo, but the active compound responsible for the antiproliferative effect is still unknown. In this study, the authors isolated and characterized the antiproliferative effect of a peptic hydrolysate of JGL2 (JGL2-H) and identified its chemical structure using MALDI-TOF-MS and MS/MS analyses. In vitro experiments using two human lung cancer cell lines, A549 and NCI-H292, showed that JGL2-H decreased the proliferation of A549 cells in a dose-dependent manner without affecting the viability of NCI-H292 cells. Among 10 major compounds in JGL2-H identified by MS/MS, only jas 0b46394aab